Description
Our Nano series Micro-Spectrophotometer measure the concentration and purity of the sample, because it is able to measure the absorbance of the whole ingredient(such as DNA, RNA, oligos and so on) of the sample at 260nm, that may make the read inaccuracy. While the fluorometer measure the fluorescence intensity of the fluorescent reagent which combine with the target molecule to read the concentration of sample. Generally, the read is lower than Nano series products but much accuracy than it._x000D_
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The sensitivity of Nano series products are above 5ng/ul, and while the fluorometer is 0.5pg/ul in measuring DNA concentration. It is important and necessary for the most applications to read the lower and accuracy concentration of the sample. Using the Fluo-100 fluorometer with the quantitation kit can measure the sample concentration rapidly, sensitively and accurately_x000D_
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Fluo-100 Simple, easy to use and applications_x000D_
The Fluo-100 is a dual-channel fluorometer, it provides high-sensitive fluorescent detection when quantifying nucleic acids and proteins, simple and cost to operate_x000D_
Applications:_x000D_
1. The sample is scarce and difficult in processing_x000D_
2. The low quantity of DNA, RNA, or protein after extraction_x000D_
3. The sample will be used in the expensive experiments: qPCR, PCR cloning, transfection, the next generation sequencing and etc._x000D_
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Two detection models of Fluo-100_x000D_
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| Code | Light | Excitation Filters | Emission Filters |
| Fluo-100A | UV | 365±20nm | 420-480nm (60nm) |
| Blue | 460±20nm | 525-570nm (45nm) | |
| Fluo-100B | Blue | 460±20nm | 525-570nm (45nm) |
| Red | 625±10nm | 670-725nm (55nm) | |
| Fluo-100C | Blue | 460±20nm | 525-570nm (45nm) |
| Green | 525±20nm | 575-640nm (65nm) |
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Fluo-100 Quantitation kit_x000D_
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High sensitivity of dsDNA Quantitation Kit: 0.2–100ng_x000D_
High sensitivity of ssDNA Quantitation Kit: 1–200ng_x000D_
Broad range of dsDNA Quantitation Kit: 100–1600ng_x000D_
Protein Quantitation Kit is researching_x000D_
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Fluo-100 Quantitation kit operation_x000D_
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Application of different fluorescence channel_x000D_
| Channel | Excitation Filters | Normal kits | Applications |
| UV channel | 365±20nm | Hoechst 33258, 4-MU,_x000D_ EnZCheK Caspase |
Nuclear acid quantification, Plant GUS reporter gene detection, Poptosis detection |
| Blue channel | 460±20nm | PicoGreen®, oligreen,RiboGreen®,_x000D_ GFP, Protein, Fluorescein, Quant-iT™ |
dsDNA, ssDNA, RNA quantification, GFP gene detection,_x000D_ Fluorescein detection, Protein detection |
| Green channel | 525±20nm | Rhodamine, Cy3,_x000D_ RFP Vybrant Cytotoxicity |
Rhodamine detection, Cy-3 fluorescence labeling detection,_x000D_ RFP gene detection, Cell activity poison detection |
| Red channel | 625±10nm | Cy5, Quant-iT RNA | Cy-5 fluorescence labeling detection, RNA quantification |
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Ordering information_x000D_
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| Code | Description | Code | Description |
| AS-18010-00 | Fluo-100A Fluorometer, DC12V 1A | RS-FL0101-S_x000D_ RS-FL0101-M_x000D_ RS-FL0101-L |
High-sensitivity dsDNA Quantitation kits (100 assays)_x000D_ High-sensitivity dsDNA Quantitation kits (200 assays)_x000D_ High-sensitivity dsDNA Quantitation kits (1000 assays) |
| AS-18020-00 | Fluo-100B Fluorometer, DC12V 1A | ||
| AS-18030-00 | Fluo-100C Fluorometer, DC12V 1A | RS-FL0102-S_x000D_ RS-FL0102-M_x000D_ RS-FL0102-L |
Broad-range dsDNA Quantitation kits (100 assays)_x000D_ Broad-range dsDNA Quantitation kits (200 assays)_x000D_ Broad-range dsDNA Quantitation kits (1000 assays) |
| AS-18011-01 | Adapter for 0.5ml qPCR tube (Standard) | ||
| AS-18011-02 | Adapter for 0.2ml qPCR tube (Options) | RS-FL0103-S_x000D_ RS-FL0103-M_x000D_ RS-FL0103-L |
High-sensitivity ssDNA Quantitation kits (100 assays)_x000D_ High-sensitivity ssDNA Quantitation kits (200 assays)_x000D_ High-sensitivity ssDNA Quantitation kits (1000 assays) |
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Fluo-100 Highlights_x000D_
1. Simple – 4.3 inch touch screen, small and easy to use_x000D_
2. Easy measurement within 3 sec for DNA, RNA, and protein_x000D_
3. High sensitivity – Lowest DNA detection limit is 0.5pg/ul_x000D_
4. Two optical channels – Equipped with two fluorescence channels for nucleic acid, protein quantitation in one detection_x000D_
5. Linear Dynamic Range–Five orders of magnitude._x000D_
6. Opening system–You can match the instrument with the reagent you choose_x000D_
7. It can save at most 1,000 data and output data by USB port_x000D_
8. Adapter: 0.5ml qPCR tube adapter 0.2ml qPCR tube adapter_x000D_
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Technical Data_x000D_
| Light source | LED |
| Dynamic range | Five orders of magnitude |
| Linear Dynamic Range | R >0.995 |
| Detector | Photodiode |
| Repeatability | <1.5% |
| Stability | <1.5% |
| Sensitivity | dsDNA: 0.5ng/ml |
| Measurement speed | 3s (Once) |
| Dimension (W×D×H)mm | 194×155×72.5 mm |
| Weight (kg) | 0.4Kg |
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